Journal: Frontiers in Immunology

Article Title: Epigenetic therapy reprograms M2-type tumor-associated macrophages into an M1-like phenotype by upregulating miR-7083-5p

doi: 10.3389/fimmu.2022.976196

Figure Lengend Snippet: Conditioned medium (CM) of M2-type macrophages treated with the combination of 5-aza-dC and TSA inhibits tumor cell survival and sensitizes tumor cells to chemotherapy. (A) Cell viability of 4T1 tumor cells after treatment with 50 nM 5-aza-dC (referred as A) and 25 nM TSA(referred as T), alone or in combination (A+T), for 24–72 (h) (B) Cell viability of 4T1 tumor cells after incubation with the CM of M2-macrophages (M2) treated with 5-aza-dC (M2A) and TSA (M2T), alone or in combination (M2AT), in the absence (blue bars) or presence of 1 μM paclitaxel (PTX, red bars), for 24 h. Black bars represent untreated (-) and PTX-treated tumor cells in the absence of CM. The CM of M1-macrophages (M1) was used as a control. Data represent the mean ± SD of three separate experiments performed in triplicates. *, P <0.05; ***, P <0.001; n.s., not significant, as assessed using one-way ANOVA followed by Tukey’s multiple comparison post-hoc test (A) or two-way ANOVA followed by Bonferroni multiple comparisons post-hoc test (B) .

Article Snippet: To examine the effect of the conditioned medium (CM) of cultured macrophages, tumor cells were incubated with the CM of M2 macrophages in the absence or presence of 1 μM of paclitaxel for 24 h. After treatment, the cells were incubated with a fresh culture medium containing 10% Cell Counting Kit-8 solution (Dojindo, Kumamoto, Japan) for 1–4 h. Absorbance was measured at the wavelength of 450 nm and cell viability was calculated based on the absorbance.

Techniques: Incubation, Control, Comparison